Technical Data
Media
Dulbecco’s MEM (DMEM) Ham’s F-12 3:1 (Powder)
D9811-14D
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:mg/L
Cupric Sulfate•5H2O0.000625
Ferric Nitrate•9H2O0.075
Ferrous Sulfate•7H2O0.2085
Magnesium Chloride, Anhyd14.39
Magnesium Sulfate, anhy73.2525
Potassium Chloride356
Sodium Chloride6699.75
Sodium Phosphate Dibasic, Anhy35.51
Sodium Phosphate Monobasic•H2O93.75
Zinc Sulfate•7H2O0.21575
Amino Acids:
L-Alanine2.25
L-Arginine•HCl115.75
L-Asparagine•H2O3.7525
L-Aspartic Acid3.325
L-Cysteine•HCl•H2O8.75
L-Cystine•2HCI47.25
L-Glutamic Acid3.675
L-Glutamine474.5
Glycine24.3775
L-Histidine•HCl•H2O36.74
L-Isoleucine79.735
L-Leucine82.025
L-Lysine•HCl118.625
L-Methionine23.62
L-Phenylalanine50.74
L-Proline8.625
L-Serine34.125
L-Threonine74.225
L-Tryptophan12.51
L-Tyrosine•Na2•2H2O79.945
L-Valine73.425
Vitamins:
D-Biotin0.001825
Choline Chloride6.49
Vitamin B-12 (Cyanocobalamin)0.34
Folic Acid3.33
myo-Inositol(I-Inositol)9.9
Niacinamide3.00925
D-Calcium Pantothenate3.12
Pyridoxine•HCl3.0155
Riboflavin0.3095
Thiamine•HCl3.085
Other:
Thymidine0.1825
D-Glucose3825.5
Hypoxanthine1.02
Linoleic Acid 0.021
ThiocticAcid (Lipoic acid)0.0525
Phenol Red11.575
Putrescine•2HCl0.04025
Sodium Pyruvate27.5
Total:12.53g/L
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM “High Glucose“ with 4500mg/ml aides in the cultivation of certain cell types. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Ham's Nutrient Mixtures were originally developed to support growth of several clones of Chinese hamster ovary (CHO) cells, of HeLa and of mouse L-cells. Ham’s mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham’s F-12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using CHO cells.


Directions per Liter: Dissolve 12.53g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2.8 to 3.7 g sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light orange to orange, homogeneous, free flowing powder

Solubility: Orange to salmon, clear, complete

pH: As reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Barnes, D., Sato, G. Analytical Biochemistry 102: 255-270 (1980). 2. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 3. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus 12: 185-196 (1960). 4. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro. 6: 89 (1970). 5. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media. In Vitro 9: 468 (1974). 6. Kaighn, M.E., (1973) in “Tissue Culture Methods and Applications,” Kruse, Jr., 7. P.F. and Patterson, Jr., M.D., eds., Academy Press, NY, 54-58. 8. Robey, P.G. and Termine, J.D. (1985) Calcif. Tissue Int. 37:453-460.