Technical Data
Media
Dulbecco’s MEM (DMEM) Ham’s F-12 w/HEPES w/o Phenol Red (Powder)
D9811-20
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•2H20154.520
Cupric Sulfate•5H2O0.001245
Ferric Nitrate•9H2O0.050
Ferrous Sulfate•7H2O0.417
Magnesium Chloride28.660
Magnesium Sulfate48.84
Potassium Chloride311.800
Sodium Chloride6996
Sodium Phosphate Dibasic71.020
Sodium Phosphate Monobasic54.300
Zinc Sulfate•7H2O0.4315
Amino Acids:
L-Alanine4.450
L-Arginine121.97
L-Asparagine•H2O7.500
L-Aspartic Acid6.650
L-Cysteine•HCI•H2O17.65
L-Cystine•2HCI31.29
L-Glutamic Acid7.350
L-Glutamine365.1
Glycine18.750
L-Histidine HCl•H2O31.48
L-Isoleucine54.47
L-Leucine59.05
L-Lysine•HCI91.25
L-Methionine17.238
L-Phenylalanine35.478
L-Proline17.250
L-Serine26.250
L-Threonine53.450
L-Tryptophan9.020
L-Tyrosine•2Na•2H2055.790
L-Valine52.850
Vitamins:
Biotin0.00365
Choline Chloride8.980
Vitamin B120.679
Folic Acid2.660
i-Inositol12.600
Nicotinamide2.018
D-Pantothenic Acid•Ca2.400
Pyridoxal HCI2.000
Pyridoxine HCI0.031
Riboflavin0.219
Thiamine HCI2.170
Other:
Thymidine0.365
D-Glucose3150
HEPES Free Acid3574
Hypoxanthine2.100
Linoleic Acid0.042
Lipoic Acid0.105
Putrescine•2HCl0.081
Sodium Pyruvate55.050
Total:15.566g/liter
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM “High Glucose“ with 4500mg/ml aides in the cultivation of certain cell types. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.

Ham's Nutrient Mixtures were originally developed to support growth of several clones of Chinese hamster ovary (CHO) cells, of HeLa and of mouse L-cells. Ham’s mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham’s F-12 was developed for growth of primary rat hepatocytes and rat prostate epithelial cells. It is also used in a clonal toxicity assay using CHO cells.


Directions per Liter: Dissolve 15.57 grams in 900 mL of distilled/deionized (DDI) water, stirring gently until completely solubilized. Do NOT heat. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Filter-sterilize using a 0.22 micron membrane. Add additional water to bring the solution to final volume. Aliquot into sterile containers.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Off white, homogeneous, free flowing powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R. and Freeman, G. Virology. 8, 396-397. (1959). 2. Smith, J.D., Freeman,G., Vogt, M. and Dulbecco, R. The Nucleic Acid of Polyoma Virus. 12, 185-196. (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro. 6, 89. (1970). 4. Rutzky, L.P. and Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro. 9, 468. (1974).