Technical Data
Media
Dulbecco’s MEM (DMEM) High Glucose w/o L-Glutamine, Methionine, Phenol Red (Powder)
D9812-06A
Cell Culture Grade
Storage RT    Shipping RT
Components shown as mg/Liter
Inorganic Salts:
Calcium Chloride•2H2O265
Ferric Nitrate•9H2O0.1
Magnesium Sulfate97.67
Potassium Chloride400
Sodium Chloride6400
Sodium Phosphate Monobasic•H2O125
Amino Acids:
L-Arginine•HCl84
L-Cystine•2HCl62.6
Glycine30
L-GlutamineAbsent
L-Histidine•HCl•H2O42
L-Isoleucine105
L-Leucine105
L-Lysine•HCl146
L-MethionineAbsent
L-Phenylalanine66
L-Serine42
L-Threonine95
L-Tryptophan16
L-Tyrosine•2Na•2H2O103.79
L-Valine94
Vitamins:
Choline Chloride4
Folic Acid4
myo-Inositol7.2
Niacinamide4
D-Pantothenic Acid•Ca4
Pyridoxine•HCl4
Riboflavin0.4
Thiamine•HCl4
Other:
D+Glucose4500
Pyruvic Acid, Sodium110
Phenol Red, NaAbsent
Total:12.921g/Liter
Many modifications of Eagle’s Medium have been developed since the original formulation appeared in literature. Among the most widely used of these modifications is Dulbecco’s Modified Eagle’s Medium (DME). DME is a modification of Basal Medium Eagle (BME) that contains a four-fold higher concentration of amino acids and viatamins, as well as additional supplementary components. The original DME formula contains 1000mg/L of glucose and was first reported for culturing embryonic mouse cells. A further alteration with 4500mg/L glucose has proved to be optimal in cultivating certain cell types.


Directions per Liter: Dissolve 12.921g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light yellow to tan free flowing powder

Solubility (12.9g/L): Clear, pale yellow, complete

pH: 6.2 ± 0.4

Endotoxin: ~10EU/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).