Technical Data
Media
Dulbecco’s MEM (DMEM) w/ HEPES w/o L-Glutamine (Powder)
D9813-05
Cell Culture Grade
Storage RT    Shipping RT
Components shown in mg/L
Inorganic Salts:
Calcium Chloride•2H2O264.9
Ferric Nitrate•9H2O0.1
Magnesium Sulfate97.7
Potassium Chloride400
Sodium Chloride6400
Sodium Phosphate Monobasic•H2O125
Amino Acids:
L-Arginine•HCl84
L-Cystine48
Glycine30
L-Histidine•HCl•H2O42
L-Isoleucine104.8
L-Leucine104.8
L-Lysine•HCl146.2
L-Methionine30
L-Phenylalanine66
L-Serine42
L-Threonine95.2
L-Tryptophan16
L-Tyrosine72
L-Valine93.6
Vitamins:
Choline Chloride4
FolicAcid4
i-Inositol7
Nicotinamide4
D-Pantothenic Acid•Ca4
Pyridoxal•HCl4
Riboflavin0.4
Thiamine•HCl4
Other:
D-Glucose4500
Pyruvic Acid, Sodium110
Phenol Red, Sodium15
Hepes4766
Total:17.7g/L
Dulbecco’s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.


Directions per Liter Dissolve 17.7g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Homogenous, free flowing powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R., Freeman, G., Virology 8: 396-397 (1959). 2. Smith, J.D., Freeman, G., Vogt, M., Dulbecco, R., The Nucleic Acid of Polyoma Virus. Virology 12(2): 185-196 (1960). 3. Morton, H.J., A Survey of Commercially Available Tissue Culture Media. In Vitro 6: 89. (1970). 4. Rutzky, L.P., Pumper, R.Q., Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro 9(6): 468-9 (1974, May-Jun).