Dulbecco’s Phosphate Buffered Saline (PBS) w/CaCl2, MgCl2 (Powder)
Cell Culture Grade
Storage RT/4°C Shipping RT
Components in g/L
Dulbecco's Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromotographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process.
D9819: Dulbecco’s Phosphate Buffered Saline (PBS) (Powder)
D9820: Dulbecco’s Phosphate Buffered Saline (PBS) w/o CaCl2, MgCl2 (Powder)
D9820-04: Dulbecco’s Phosphate Buffered Saline (PBS), Modified w/NaCl, NaH2PO4 w/o KCl, CaCl2, MgCl2 (Powder)
D9820-05: Dulbecco’s Phosphate Buffered Saline 10X (PBS) w/o CaCl2, MgCl2 (Powder)
D9821: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) (Powder)
D9822: Dulbecco’s Phosphate Buffered Saline (PBS) w/EDTA, w/o CaCl2, MgCl2 (Powder)
D9823: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) w/MgSO4 (Powder)
Directions per Liter: Dissolve 9.783 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Appearance: White, homogeneous, free flowing powder
Solubility: Colorless, clear, complete
pH: As Reported
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Dulbecco, R., Vogt, M.: Plaque Formation and Isolation of Pure Lines with Poliomyelitis Viruses. J. Exp. Med. 106: 167-169 (1957).