Technical Data
Media
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Technical Data
Media
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Dulbecco’s Phosphate Buffered Saline (PBS), Modified w/NaCl, NaH2PO4 w/o KCl, CaCl2, MgCl2 (Powder)
D9820-04
Cell Culture Grade
Storage RT Shipping RT
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Components in mg/L
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Dulbecco's Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromotographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process. Also Available: D9819: Dulbecco’s Phosphate Buffered Saline (PBS) (Powder) D9820: Dulbecco’s Phosphate Buffered Saline (PBS) w/o CaCl2, MgCl2 (Powder) D9820-04: Dulbecco’s Phosphate Buffered Saline (PBS), Modified w/NaCl, NaH2PO4 w/o KCl, CaCl2, MgCl2 (Powder) Dulbecco’s Phosphate Buffered Saline 10X (PBS) w/o CaCl2, MgCl2 (Powder) D9821: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) (Powder) D9822: Dulbecco’s Phosphate Buffered Saline (PBS) w/EDTA, w/o CaCl2, MgCl2 (Powder) D9823: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) w/MgSO4 (Powder) Directions per Liter (1X): Dissolve 9.230 grams per liter of distilled/deionized (DDI) water. Stir until completely dissolved. Adjust pH as necessary. May be filter-sterilized or autoclaved for 15 minutes at 121ºC (15psi). Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time. Appearance: White, homogeneous, free flowing powder Solubility: Colorless, clear, complete pH: 6.95 ± 0.4 Endotoxin: ~10Eu/mg Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Dulbecco, R., Vogt, M.: Plaque Formation and Isolation of Pure Lines with Poliomyelitis Viruses. J. Exp. Med. 106: 167-169 (1957). |