Technical Data
Media
Dulbecco’s Phosphate Buffered Saline (PBS), Modified w/NaCl, NaH2PO4 w/o KCl, CaCl2, MgCl2 (Powder)
D9820-04
Cell Culture Grade
Storage RT    Shipping RT
Components in mg/L
Sodium Chloride7650.000
Sodium Phosphate dibasic1268.800
Sodium Phosphate monobasic 100.000
Potassium Phosphate monobasic211.300
Total:9.230g/L
Dulbecco's Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromotographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process.

Also Available:
D9819: Dulbecco’s Phosphate Buffered Saline (PBS) (Powder)
D9820: Dulbecco’s Phosphate Buffered Saline (PBS) w/o CaCl2, MgCl2 (Powder)
D9820-04: Dulbecco’s Phosphate Buffered Saline (PBS), Modified w/NaCl, NaH2PO4 w/o KCl, CaCl2, MgCl2 (Powder)
Dulbecco’s Phosphate Buffered Saline 10X (PBS) w/o CaCl2, MgCl2 (Powder)
D9821: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) (Powder)
D9822: Dulbecco’s Phosphate Buffered Saline (PBS) w/EDTA, w/o CaCl2, MgCl2 (Powder)
D9823: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) w/MgSO4 (Powder)


Directions per Liter (1X): Dissolve 9.230 grams per liter of distilled/deionized (DDI) water. Stir until completely dissolved. Adjust pH as necessary. May be filter-sterilized or autoclaved for 15 minutes at 121ºC (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White, homogeneous, free flowing powder

Solubility: Colorless, clear, complete

pH: 6.95 ± 0.4

Endotoxin: ~10Eu/mg


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R., Vogt, M.: Plaque Formation and Isolation of Pure Lines with Poliomyelitis Viruses. J. Exp. Med. 106: 167-169 (1957).