Technical Data
Dulbecco’s Phosphate Buffered Saline 10X (PBS) w/o CaCl2, MgCl2 (Powder)
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components in mg/L (10X):
Potassium Chloride2000.000
Potassium Phosphate mono2000.000
Sodium Chloride80000.000
Sodium Phosphate dibasic11500.000
Total:95.5g/L (10X)
Dulbecco's Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromotographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process.

Also Available:
D9819: Dulbecco’s Phosphate Buffered Saline (PBS) (Powder)
D9820: Dulbecco’s Phosphate Buffered Saline (PBS) w/o CaCl2, MgCl2 (Powder)
D9820-04: Dulbecco’s Phosphate Buffered Saline (PBS), Modified w/NaCl, NaH2PO4 w/o KCl, CaCl2, MgCl2 (Powder)
Dulbecco’s Phosphate Buffered Saline 10X (PBS) w/o CaCl2, MgCl2 (Powder)
D9821: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) (Powder)
D9822: Dulbecco’s Phosphate Buffered Saline (PBS) w/EDTA, w/o CaCl2, MgCl2 (Powder)
D9823: Dulbecco’s Phosphate Buffered Saline (PBS) (10X) w/MgSO4 (Powder)

Directions per Liter: Dissolve 95.5 grams in ~950ml of ddH2O, stirring gently with heating until completely solubilized. Allow to cool and then adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Solution may be filter-sterilized or autoclaved for 15 minutes at 121ºC (15psi). Bottles of stored solution can be warmed in a hot water bath prior to use if crystallization is observed.

USB recommends the preparation of this product as a 1X solution. Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: White, homogeneous, free flowing powder

Solubility: Colorless, clear, complete

pH: As Reported

Endotoxin: 1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Dulbecco, R., Vogt, M.: Plaque Formation and Isolation of Pure Lines with Poliomyelitis Viruses. J. Exp. Med. 106: 167-169 (1957).