Technical Data
D9900-01
Dynamin
Description:
Dynamin is a 100kD GTP binding protein that was first identified as a protein able to bind microtubules. A mutation in the Drosophila gene shits, encoding a dynamin homolog, prevents synaptic vesicle recycling and causes temperature-sensitive paralysis, implicating dynamin in the formation of coated vesicles. Dynamin may mediate vesicle formation by assembling into a ring structure around the necks of invaginated coated pits that may eventually lead to the closure of the neck and formation of vesicles. Amphiphysin, dynamin, clathrin and AP2 form a complex during synaptic vesicle endocytosis, which is regulated by the phosphorylated state of different components in this comple. To date, three genes have been found to encode dynamin isoforms. Dynamin 1 is neuronal specific. Dynamin 2 is ubiquitously expressed. Dynamin 3 localizes to testis, brain and lung.

Applications:
Suitable for use in Western Blot, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot (Colorimetric): 0.5ug/ml
Flow Cytometry: 10ug/ml
Immunohistochemistry: 1:50; Stains human colon cancer tissue. Staining of formaldehyde perfused and fixed rat brain shows synaptic immunofluorescence.
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse Brain Tissue Extract

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG14E67Affinity Purified
SizeStorageShippingSourceHost
25ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant His 6-tagged human dynamin (aa1-750). Epitope: GNRGMEDLIPLVNRLQD.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1mM PMSF, 50% glycerol.
Specificity:
Recognizes a 100kD protein, corresponding to human dynamin. Species Crossreactivity: mouse, rat, chicken and bovine. Sequence Homology: Dynamin and Dynamin 1 at 17/17 residues (aa2-18) and dynamin 2 and 3 at 15/17 residues.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Atapattu, D.N. and Czuprynski, C.J. (2007) Infection and Immunity 75:4719-4727. 1. Shpetner, H.S. & Vallee, R.B., Cell 59: 421-432 (1989). 2. van der Bliek, A.M. & Meyerowitz, E.M., Nature 351: 411-414 (1991). 3. Takei, K., et al., Nature 374: 186-190 (1995). 4. Slepnev, V.I., et al., Science 281: 821-824 (1998). 5. Cao, H., et al., Mol. Biol. Cell. 9: 2595-2609 (1998).