Technical Data
Dynorphin A
Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin.
Cells are found in the paraventricular and supraoptic nucleus and fibers are found in the lateral hypothalamus in rat brain of 4% FA perfused colchicine tissue.

Suitable for use in RID and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
RID: 1:10,000
Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining. Sodium citrate buffer, pH 6.0, is recommended. Overnight incubation recommended.
Immunohistochemistry (Frozen): 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ul-20CBlue IcePorcineRabbit
Not determined
Synthetic peptide corresponding to aa209-225, YGGFLRRIRPKLKWDNQ of porcine Dynorphin A. Cellular Localization: Secreted. Species Sequence Homology (100%): mouse, bovine, guinea pig.
Supplied as a liquid in PBS, 0.1% BSA, 0.09% sodium azide.
Recognizes porcine Dynorphin A. Blocks dynorphin 1-17 and 1-8 but not with other related peptides. Species Crossreactivity: human, rat, rhesus monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Beck, H., et al., J. Neurosci. 20: 7080-7086 (2000). 2. Sherman, T.G., et al., J. Neurosci. 8: 3785-3796 (1988). 3. Zardetto-Smith, A.M., et al., Peptides 9: 1121-1127 (1988). 4. Khachaturian, H., et al., Peptides 6(2):155-166 (1985).