Technical Data
D9910X
Dystrophin (BSA & Azide Free)
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Technical Data |
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D9910X |
Dystrophin (BSA & Azide Free) |
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Description: Dystrophin is a member of the spectrin/alpha-actinin family of actin binding, triple helix rod containing proteins. It is absent or greatly reduced in individuals withe the X-linked recessive Duchenne’s muscular dystrophy disorder, as well as in mice with the mdx, (murine muscular dystrophy) mutation. Applications: Suitable for use in Affinity Purification, Electron Microscopy, ELISA, Immunofluorescence and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (Formalin/Paraffin): 2-4ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Note that 1mM EDTA, pH 8.0 is better than 10mM citrate buffer, pH 6.0 for unmasking the epitope. Optimal dilutions to be determined by the researcher. Positive Control: Skeletal muscle Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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