Technical Data
E2F1 Transcription Factor (E2F 1, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, PBR3, RBAP-1, RBBP3, RBP3)
Cellular Localization: Nuclear

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide: KTPSQEVTSEEENRATDSAT conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 310-329 of Human E2F 1.
Purified by immunoaffinity chromatography.
10mM PBS. [pH 7.4] with 1% BSA containing 15mM Sodium Azide
Species Crossreactivity: Crossreacts with Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Cartwright P et al. E2F-6: a novel member of the E2F family is an inhibitor of E2F-dependent transcription. Oncogene 17:611-23 (1998). Rogers KT et al. DP-2, a heterodimeric partner of E2F: identification and characterization of DP-2 proteins expressed in vivo. Proc Natl Acad Sci U S A 93:7594-9 (1996). Moberg K et al. E2F-4 switches from p130 to p107 and pRB in response to cell cycle reentry. Mol Cell Biol 16:1436-49 (1996). Hateboer G et al. Degradation of E2F by the ubiquitin-proteasome pathway: regulation by retinoblastoma family proteins and adenovirus transforming proteins. Genes Dev 10:2960-70 (1996).