Technical Data
E0010-02P
E2F1, phosphorylated (Ser332) (Transcription Factor E2F1, E2F-1, Retinoblastoma Binding Protein 3, RBBP-3, PRB-binding Protein E2F-1, PBR3, Retinoblastoma-associated Protein 1, RBAP-1, RBBP3)
Description:
E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Applications:
Suitable for use in ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser332 of human E2F1 (KLH).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human E2F1 when phosphorylated at Ser332.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.O'Donnell, K.A., et al., Nature 435(7043):839-843 (2005). 2.Wang, C., et al., J. Biol. Chem. 280(13):12339-12343 (2005). 3.Joshi, B., et al., Oncogene 24(13):2204-2217 (2005). 4.Saberwal, G., et al., Int. J. Hematol. 80(2):146-154 (2004). 5.Chaussepied, M., et al., Mol. Cell 16(5):831-837 (2004).