Technical Data
Media
Earle’s Balanced Salts (10X) (Powder)
E0253
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Calcium Chloride•H2O2649
D-Glucose10000
Magnesium Sulfate•7H2O2000
Phenol Red100
Potassium Chloride4000
Sodium Chloride68000
Sodium Phosphate Mono•H2O1400
Total:88.2g/liter
The search for a synthetic medium to replace serum for maintaining cells in vitro began in the late nineteenth century and continues to this day. Ringer, Locks and Tyrode substituted physiological salt solutions augmented with glucose for serum and thereby laid the foundation for the development of defined media. As biochemical and analytical techniques have improved, more of the components in serum such as vitamins, hormones, and amino acids have been identified and incorporated into physiological salt solutions, reducing, and in some cases eliminating the concentrations of animal sera required as a medium supplement. Although there have been many modifications to the original formulas in efforts to produce fully defined media, salt solutions still play an important role in tissue culture. A salt solution's basic function, to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions, is as valuable today as when it was first developed a century ago. Earle’s Balanced Salts is one of the balanced salts available from USBio. The selection of a balanced salt is strongly influenced by 1. type of cell, 2. type of culture monolayer, suspension, clonal, and 3. degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.


Directions per Liter: Dissolve 88.2g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 22 sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter sterilize using a 0.22 micron membrane filter.

Note: Some precipitate may be observed due to excess concentration of salts. USB recommends the preparation of this product as a 1X solution.Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Light orange to pink, homogeneous, free flowing powder

Solubility (10X): Yellow, clear with possible precipitate.

Solubility (1X): Yellow, clear, complete

pH (10X): As Reported

pH (1X): As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.