Technical Data
EAT-4 (Vesicular Type Transporters for Glutamate, VGLUT)
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000 for antiserum and 1-10ug/ml for affinity pure antibody using Chemiluminescence technique. EAT-4 is ~65kD protein.
ELISA: 1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity pure. Control peptide can be used to coat ELISA plates at 1ug/ml.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ul-20CBlue IceRatRabbit
As reported
An 18 AA synthetic peptide witin the N-terminus of C. elegans EAT-4 was synthesized, conjugated to KLH
Affinity purified over the control-peptide Sepharose
Supplied as a liquid in PBS, pH 7.4 and 0.09% sodium azide, 40% glycerol.
The peptide has no significant sequence with mammalian VGLUT1/2 or other NaPi-related (Type-1 to Type-III) transporters. Antibody crossreactivity in various species is not established. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking (use 5-10 ug per 1 ul of antiserum or 1 ug of aff pure IgG) to confirm antibody specificity
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lee RYN et al (1999) J. Neurosci. 19:159-167.