Technical Data
E1050
EBP50 (ERM (Ezrin-Radixin-Moesin) Binding Phosphoprotein of 50kD, Na(+)/H(+) Exchange Regulatory Cofactor NHE-RF1, NHERF, NHERF1, NHERF-1, NPHLOP2, Regulatory Cofactor of Na(+)/H(+) Exchanger, Sodium Hydrogen Exchanger Regulatory Factor 1, Solute Carrier
Description:
EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50kD] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes. EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence/Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml
Immunohistochemistry (paraffin, frozen): 10ug/ml Staining of EBP50 in mouse airway results in staining of the epithelium.
Immunofluorescence (IC): 1ug/ml
Immunoprecipitation: 1:10-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant human His-tagged EBP 50 obtained from bacteria. All His-tag specific antibodies have been removed.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide. Also available with Biotin and without BSA or azide. See E1050B.
Specificity:
Recognizes EBP 50 from human T84 and Calu3 cells as well as recombinant human protein at ~50kD. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Fouassier L, et al., (2001) Hepatology 33:166-76. 2.Fouassier L, et al., (2000) J Biol Chem 275:25039-45. 3. Bretscher A, et al., (2000) Annu Rev Cell Dev Biol 16:113-43.