		Technical Data
E2000-25H	ECSIT, CT (Evolutionarily Conserved Signaling Intermediate in Toll Pathway, Evolutionarily Conserved Signaling Intermediate in Toll Pathways, ECSIT Homolog, ECSIT Protein, Signaling Intermediate in Toll Pathway Evolutionarily Conserved, SITPEC, SITPEC Pro

Description:
Activation of NF-kB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 to kinases such as IRAK and other intermediates such as TNF receptor associated factor (TRAF)- 6. ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis, indicating the possibility of cross-talk between the TLR/IL-B and Bmp signaling pathways.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Highly Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to 14aa near the C-terminus of human ECSIT.

Purity:

Purified by Ion Exchange chromatography.

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

Specificity:

Recognizes human ECSIT. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Takeda, K. et al. Annu. Rev. Immunol. 21, 335 (2003). 2. Vogel, SN. et al. Mol. Interv. 3, 466(2003). 3. Janssens, S. et al. Mol. Cell. 11, 293 (2003). 4. Sato, S. et al. J. Immunol. 171(8), 4304 (2003). 5. Kopp, E. et al. Genes Dev. 13, 2059 (1999). 6. Xiao, C. et al. Genes Dev. 17, 2933 (2003).