Technical Data
EDD, CT (E3 Ubiquitin-protein Ligase UBR5, E3 Ubiquitin-protein Ligase, HECT Domain-containing 1, Hyperplastic Discs Protein Homolog, hHYD, Progestin-induced Protein, UBR5, EDD1, HYD, KIAA0896)
EDD is a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22, a locus disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.

Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
Synthetic peptide selected from the C-terminal region of human EDD (KLH).
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human EDD. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Clancy, J.L., et al., Oncogene 22(32):5070-5081 (2003). 2. Henderson, M.J., et al., J. Biol. Chem. 277(29):26468-26478 (2002). 3. Honda, Y., et al., J. Biol. Chem. 277(5):3599-3605 (2002). 4. Callaghan, M.J., et al., Oncogene 17(26):3479-3491 (1998).