Technical Data
Media
Edinburgh Minimal Media, Nitrogen Free (EMM2) (Powder)
E2205-01
Molecular Biology Grade
Storage RT    Shipping RT
Components shown as g/Liter
Base:
Potassium Hydrogen Phthalate3.00
Sodium Phosphate Dibasic2.20
Ammonium ChlorideAbsent
Glucose20.00
Salt Stock:
Magnesium Chloride•6H2O1.05
Calcium Chloride•2H2O0.0147
Potassium Chloride1.00
Sodium Sulfate0.04
Vitamin Stock:
Pantothenic Acid0.001
Nicotinic Acid0.01
Inositol0.01
Biotin0.00001
Mineral Stock:
Boric Acid0.0005
Manganese Sulfate0.0004
Zinc Sulfate•7H2O0.0004
Ferrous Chloride0.0002
Molybdic Acid0.00004
Potassium Iodide0.0001
Copper Sulfate •5H2O0.00004
Citric Acid0.001
Total:27.33g/Liter
Note:
The above formulation was derived from the following referenced formula:
Edinburgh Minimal Medium (EMM 2)
3g/L Potassium hydrogen phthalate (14.7mM)
2.2g/L Sodium phosphate dibasic (15.5mM)
5g/L Ammonium chloride (93.5mM)
2% (w/v) Glucose(111mM)
20ml/L Salts (50X stock)
1ml/L Vitamins (1000X stock)
0.1ml/L Minerals (10,000X stock)
50X Salts:
52.5g/L Magnesium chloride•6H2O (0.26M)
0.735g/L Calcium chloride•2H2O (4.99mM)
50g/L Potassium chloride (0.67M)
2g/L Sodium sulfate (14.1mM)
1000X Vitamins:
1g/L Pantothenic acid (4.20mM)
10g/L Nicotinic acid (81.2mM)
10g/L Inositol (55.5mM)
10mg/L Biotin (40.8uM)
10,000X Minerals:
5g/L Boric acid (80.9mM)
4g/L Manganese sulfate (23.7mM)
4g/L Zinc sulfate•7H2O (13.9mM)
2g/L Ferrous Chloride (7.40mM)
0.4g/L Molybdic acid (2.47mM)
1g/L Potassium iodide (6.02mM)
0.4g/L Cupric sulfate.5H2O (1.60mM)
10g/L Citric acid (47.6mM)
Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomyces pombe. EMM2 is used for vegetative growth. This formulation does not contain Ammonium Chloride, a Nitrogen source.

Appearance:
White to light tan, homogeneous, free flowing powder

Solubility:
Colorless, clear, complete

pH: As Reported


Directions per Liter: Dissolve 27.33g in 800-900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.