Technical Data

Media

Edinburgh Minimal Media Glucose Free (Powder)

E2205-11

Molecular Biology Grade

Storage RT/4°C Shipping RT

Components shown as g/liter


Base:
Ammonium Chloride	5.00
Potassium Hydrogen Phthalate	3.00
Sodium Phosphate Dibasic	2.2
Glucose	Absent

Salt Stock:
Calcium Chloride•2H2O	0.0147
Magnesium Chloride•6H2O	1.05
Potassium Chloride	1.00
Sodium Sulfate	0.04

Vitamin Stock:
Biotin	0.00001
Inositol	0.01
Nicotinic Acid	0.01
Pantothenic Acid	0.001

Mineral Stock:
Boric Acid	0.0005
Citric Acid	0.001
Copper Sulfate •5H2O	0.00004
Ferrous Chloride	0.0002
Manganese Sulfate	0.0004
Potassium Iodide	0.0001
Molybdic Acid	0.00004
Zinc Sulfate•7H2O	0.0004

Total:	12.328g/liter


Note:
The above formulation was derived from the following referenced formula:

Edinburgh Minimal Medium (EMM 2)
3g/L Potassium hydrogen phthalate (14.7mM)
2.2g/L Sodium phosphate dibasic (15.5mM)
5g/L Ammonium chloride (93.5mM)
2% (w/v) Glucose(111mM)
20ml/L Salts (50X stock)
1ml/L Vitamins (1000X stock)
0.1ml/L Minerals (10,000X stock)

50X Salts:
52.5g/L Magnesium chloride•6H2O (0.26M)
0.735g/L Calcium chloride•2H2O (4.99mM)
50g/L Potassium chloride (0.67M)
2g/L Sodium sulfate (14.1mM)

1000X Vitamins:
1g/L Pantothenic acid (4.20mM)
10g/L Nicotinic acid (81.2mM)
10g/L Inositol (55.5mM)
10mg/L Biotin (40.8uM)

10,000X Minerals:
5g/L Boric acid (80.9mM)
4g/L Manganese sulfate (23.7mM)
4g/L Zinc sulfate•7H2O (13.9mM)
2g/L Ferrous Chloride (7.40mM)
0.4g/L Molybdic acid (2.47mM)
1g/L Potassium iodide (6.02mM)
0.4g/L Cupric sulfate.5H2O (1.60mM)
10g/L Citric acid (47.6mM)

Edinburgh Minimal Media is a thiamine-free formulation for nmt1 promoter studies for Schizosaccharomyces pombe transformations. The Low Glucose formulation is used for protoplasting and yeast transformations.

Directions per Liter: Dissolve 12.328g in 800-900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to light tan, homogeneous, free flowing powder

Solubility: Colorless, clear, complete

pH: 6.0 ± 0.7

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.