Technical Data
E2211-50D
EED (embryonic ectoderm development, hEED, HEED, Polycomb protein EED, WAIT1, WAIT-1, WD protein associating with integrin cytoplasmic tails 1)
Description:
Polycomb group proteins are important for maintaining transcriptional silencing. One conserved PcG complex, PRC2, is composed of several proteins including the histone methyltransferase EZH2, the WD-repeat protein EED (Embryonic ectoderm development), and the Zn-finger protein Suz12. Transcriptional repression mediated by EED involves histone deacetylation, while the EZH2 methylates histone H3 on lysine 27. EED protein is present in four isoforms. These EED isoforms selectively associate with distinct EZH2-containing complexes, resulting in differential targeting of their associated methyltransferase activity. These complexes play a role in Hox gene silencing, X-inactivation, germline development, stem cell pluripotency and cancer metastasis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000
Optimal dilutions to be determined by the researcher.

Positive Control:
A431 Cell Lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a,k9H228Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant fusion protein of full length murine EED tagged with MBP.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 0.1 M Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes mouse EED (5-6 isoforms of EED are detected). Species Crossreactivity: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. The Protein Data Bank, www.pdb.org, DOI 10.2210/pdb2qxv/pdb 2. Pasini, D., et al. (2007). Mol. Cell.
Biol. 27, 3769-3779. 3. Bracken, A.P., et al. (2003). EMBO J. 22: 5323-5335. 4. Pasini, D., et al. (2004). 5. Bracken, A.P., et al. (2007). Cell Cycle. 3: 396-400 6. Bracken, A.P., et al. (2006). Genes Dev. 20: 1123-1136.