		Technical Data
E2213-98	EGFP (Enhanced Green Fluorescent Protein)

Description:
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Excitation max (nm): 488

Emission max (nm): 509

Extinction coefficient (Cm-1M-1): 61000

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice		Rabbit

Concentration:

Not Determined

Immunogen:

Recombinant EGFP

Purity:

Serum

Form

Supplied as a lyophilized powder. Reconstitute in 100ul of sterile ddH2O. Centrifuge to remove any insoluble material.

Specificity:

Recognizes EGFP.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Chalfie, M., Tu, Y. et al (1994) Science 263:802-805. 2. Tsien, R. Y. (1998) Annu Rev Biochem 67:509-544. 3. Dardalhon, V. et al (1999) Hum Gene Ther 10:5-14. 4. Kain, S. R. (1999) DDT 4:304-312.