Technical Data
E2213-98
EGFP (Enhanced Green Fluorescent Protein)
Description:
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Excitation max (nm): 488

Emission max (nm): 509

Extinction coefficient (Cm-1M-1): 61000

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceRabbit
Concentration:
Not Determined
Immunogen:
Recombinant EGFP
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute in 100ul of sterile ddH2O. Centrifuge to remove any insoluble material.
Specificity:
Recognizes EGFP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chalfie, M., Tu, Y. et al (1994) Science 263:802-805. 2. Tsien, R. Y. (1998) Annu Rev Biochem 67:509-544. 3. Dardalhon, V. et al (1999) Hum Gene Ther 10:5-14. 4. Kain, S. R. (1999) DDT 4:304-312.