Technical Data
E2230-13B
Elastase, Neutrophil (HRP)
Description:
Elastase, like trypsin and chymotrypsin, is a serine protease that also hydrolyses amides and esters It is produced in the pancreas as an inactive zymogen, proelastase, and activated in the duodenum by trypsin. Elastase is distinctive in that it acts upon elastin.

Applications:
Suitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Rz ratio (Reinheitszahl, A403/A280): 0.27

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanSheep
Concentration:
~2mg/ml
Immunogen:
Neutrophil elastase prepared from human sputum
Purity:
Purified by caprylic acid and ammonium sulfate precipitations, and solid-phase adsorption to remove unwanted crossreactivity before conjugation.
Form
Supplied in a buffered stabilizer solution, 50% glycerol. No preservatives are added. Labeled with horseradish peroxidase (HRP).
Specificity:
Recognizes human elastase.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.