		Technical Data
E2230-13G	Elastase, Neutrophil (ELANE, ELA2, GE, HLE, HNE, NE, PMN-E, SCN1)

Description:
The primary role of neutrophils is to destroy any foreign particles, either by phagocytosis or by release of bacteriostatic agents. Neutrophils contain high concentrations of the serine proteinases neutrophil elastase that is stored in intracellular vacuoles termed the azurophilic granules. This proteinase is a major protein constituent of neutrophils, comprising around 1-3pg per neutrophil. Their primary role is to digest any pathogens that the neutrophil has phagocytosed.

Applications:
Suitable for use in ELISA, Flow Cytometry and Functional Assays. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06D IgG, H&L, X-Adsorbed (AP) Pab Gt xMo
I1904-06J IgG, H&L, X-Adsorbed (AP) Pab Gt xMo

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	10C46	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Elastase from human neutrophils.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Specificity:

Recognizes human neutrophil elastase.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Durant, S. et al. (2004) Apoptosis-induced proteinase 3 membrane expression is independent from degranulation. J. Leukoc. Biol. 75(1): 87-98. 2. Davies, P.L. et al. (2008) Monoclonal anti-neutrophil elastase antibody characterisation: ability to block function, detect free versus serpin-complexed enzyme and stain intracellular granules. J Immunol Methods. 336: 175-82.