Technical Data
E2236-21B
Elastase, Pancreatic
Description:
Elastin is a protein in connective tissue that is elastic and allows many tissues in the body to resume their shape after stretching or contracting. Elastin helps skin to return to its original position when it is poked or pinched. Elastin is also an important load-bearing tissue in the bodies of mammals and used in places where mechanical energy is required to be stored. Elastin is particularly abundant in large elastic blood vessels such as the aorta. It is also very important in the lungs, elastic ligaments, the skin, the bladder, and elastic cartilage.
Elastin is composed largely of glycine, proline, and other hydrophobic residues and contains multiple lysine-derived crosslinks, such as desmosines, which link the individual polypeptide chains into a rubberlike network. The hydrophobic regions of the chains, between the crosslinks, are highly mobile. The hydrophobic and crosslinking domains are coded by separate, small (27 to 114 bp) exons that are separated by large introns. The initial translation product is a 72kD polypeptide, designated tropoelastin. Elastin is made by linking many soluble tropoelastin protein molecules, in a reaction catalyzed by lysyl oxidase, to make a massive insoluble, durable cross-linked array.

Applications:
Suitable for Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:200 -1:500
ELISA: 1:1000 -1:2000
Immunoprecipitation: 1:100 -1:200
Immunohistochemistry: 1:50 -1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
500ul-20CBlue IceRatRabbit
Concentration:
Not determined
Immunogen:
Rat pancreatic elastase
Purity:
Serum
Form
Supplied as a lyophilized powder. Preservative free. Reconstitute with 500ul sterile ddH2O.
Specificity:
Recognizes rat pancreatic elastase. Species Crossreactivity: mouse. Does not crossreact with porcine or human neutrophil elastase.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.