Technical Data
E2249-80
Elongator Protein 1 (ELP1, Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B-cells, Kinase Complex-associated Protein, IKBKAP, DYS, ELP1, FD, FLJ12497, IKAP, IKI3, P150, TOT1, DKFZP781H1425)
Description:
Its function is not clear, but it has been demonstrated that the human Elongator facilitates transcription by RNA polymerase II in a chromatin- and acetyl-CoA-dependent manner. IKBKAP. IKBKAP was originally described as a scaffold protein of the IKK complex involved in NF-kB activation, but this role has since been disputed. IKBKAP is a component of the RNA polymerase II elongator complex which has histone acetyltransferase activity and is involved in transcriptional elongation. Gene defects are a cause of familial dysautonomia, also known as Riley-Day syndrome. Affected individuals show a variety of symptoms such as decreased sensitivity to pain and temperature, cardiovascular instability, pneumonia and gastrointestinal dysfunction.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a9F621Affinity Purified
SizeStorageShippingSourceHost
150ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Elongator complex (130kD)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes human ELP1
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Kim, J-H. (2002) Proc. Natl. Acad. Sci. USA 99,1241 " 1246; Hawkes, N. et al. (2002) J. Biol. Chem. 277, 3047 " 3052.