Technical Data
EN2 (Engrailed-2, Engrailed Homeobox 2, Engrailed Homolog 2, AUTS1)
EN1 and EN2 encode homeodomain containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. EN2 may play a role in susceptibility to autism spectrum disorders.

Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Peptide ELISA: 1:2000
Western Blot: 0.5-1.5ug/ml. BaNone Detected at ~30kD with mouse brain lysates.
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse Brain lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide corresponding to C-NHSTTAKEGKSDSE of the C Terminus of human EN2 (NP_001418.2).
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-saline, pH 7.3. 0.5% BSA, 0.02% sodium azide.
Recognizes human EN2. Species Crossreactivity: mouse, rat and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Benayed R, et al., (2005) Am J Hum Genet. Nov;77(5):851-68. Epub Oct 5.