Technical Data
E2270-10
EN2 (Engrailed-2, Engrailed Homeobox 2, Engrailed Homolog 2, AUTS1)
Description:
EN1 and EN2 encode homeodomain containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. EN2 may play a role in susceptibility to autism spectrum disorders.

Applications:
Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Peptide ELISA: 1:2000
Western Blot: 0.5-1.5ug/ml. BaNone Detected at ~30kD with mouse brain lysates.
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse Brain lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-NHSTTAKEGKSDSE of the C Terminus of human EN2 (NP_001418.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH 7.3. 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human EN2. Species Crossreactivity: mouse, rat and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Benayed R, et al., (2005) Am J Hum Genet. Nov;77(5):851-68. Epub Oct 5.