Technical Data
E2270-11
EN2 (Homeobox Protein Engrailed-2, Homeobox Protein en-2, Hu-En-2)
Description:
EN2 is thought to have a role in controlling development. In Drosophila, the protein plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. This protein has been implicated in the control of pattern formation during development of the central nervous system.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000
Western Blot: 0.1-0.3ug/ml, observed in mouse and rat brain lysates on ~30kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-NHSTTAKEGKSDSE, from rat EN2, at C-terminal (NP_001418.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes rat EN2. Species sequence homology: Canine, human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Benayed R, Gharani N, Rossman I, Mancuso V, Lazar G, Kamdar S, Bruse SE, Tischfield S, Smith BJ, Zimmerman RA, Dicicco-Bloom E, Brzustowicz LM, Millonig JH. Support for the Homeobox Transcription Factor Gene ENGRAILED 2 as an Autism Spectrum Disorder Susceptibility Locus. Am J Hum Genet. 2005 Nov;77(5):851-68. Epub 2005 Oct 5.