Description:
SLE is a flavivirus producing severe encephalitis in humans. The viral envelope contains a single glycoprotein serving as a major structural component of the virion spike.
Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications not tested.
Recommended Dilutions:
Optimal dilutions to be determined by the researcher.
Hybridoma:
Spleen cells from immunized mice were fused with cells of the SP2/0 Ag 14 myeloma cell line.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2a | 10D421 | Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | | Mouse |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Purified SLE strain MSI-7. |
| Purity: |
| Purified |
| Form |
| Supplied as a liquid in PBS, 0.1% sodium azide. |
| Specificity: |
| Recognizes the Saint Louis Encephalitis virus strain (MSI-7) envelope glycoprotein. Crossreactivity: Japanese Encepahalitis (Nakayama), West Nile (EG101), Murray Valley Encephalitis (Original), Yellow Fever (17D), Dengue 1 (Hawaii), Dengue 2 (New Guinea C), Dengue 3 (H87) and Dengue 4 (H241). |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Roehrig, J.T. et al. (1983) Identification of epitopes on the E glycoprotein of Saint Louis encephalitis virus using monoclonal antibodies. Virology. 128: 118-126. 2. Vorndam V et al. (1993) Molecular and biological characterization of a non-glycosylated isolate of St Louis encephalitis virus. J Gen Virol. 74: 2653-2660. 3. Mathews, J.H. and Roehrig, J.T. (1984) Elucidation of the topography and determination of the protective epitopes on the E glycoprotein of Saint Louis encephalitis virus by passive transfer with monoclonal antibodies. J Immunol. 132: 1533-1537.
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