Technical Data
Endonuclease G (EndoG)
The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is speci cally activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-speci c nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the rst mitochondrial protein identi ed to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).

Applications: Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution: Western Blot: 1-2ug/ml; Optimal dilutions to be determined by the researcher.

Positive Control: HepG2 and 3T3 cell lysates

Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Raised with a synthetic peptide (GGPRGPGELAKYGLP) corresponding to amino acids 55 to 70 of human EndoG (5).
Purified by affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Recognizes a 35kD band. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Li LY, Luo X, Wang X. (2001) Nature 412(6842):959. 2. Parrish J, et al., (2001) Nature 412(6842):904. 3. Hengartner MO, (2001) Nature. 412(6842):27, 29. 4. Widlak P, et al., (2001) J Biol Chem. 276(51):484049. 5. Tiranti V, et al., (1995) Genomics 25(2):55964.