Technical Data
E2291-40A
Endonuclease G (EndoG)
Description:
Recommended Secondary Reagents: Sheep anti Rabbit IgG (Aff. Pur.): HRP

Applications:
Western Blotting: 1-2ug/ml
Optimal working dilutions to be determined by researcher.

Western Blotting: Detects a band of approximately 35kD in HepG2 and 3T3 cell lysates.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide GGPRGPGELAKYGLP corresponding to amino acids 55 - 70 of endonuclease G.
Purity:
Purified IgG was prepared from whole serum by affinity chromatography.
Form
Supplied as a liquid in PBS with 0.02% sodium azide.
Specificity:
Recognizes endonuclease G (endoG), a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis endoG i reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria. Species Crossreactivity: Human. Expected crossreactivity from sequence similarity mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Li Ly, et al., (2001) Nature 412:95-99.