Description:
Recommended Secondary Reagents: Sheep anti Rabbit IgG (Aff. Pur.): HRP
Applications:
Western Blotting: 1-2ug/ml
Optimal working dilutions to be determined by researcher.
Western Blotting: Detects a band of approximately 35kD in HepG2 and 3T3 cell lysates.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | Human | Rabbit |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Synthetic peptide GGPRGPGELAKYGLP corresponding to amino acids 55 - 70 of endonuclease G. |
| Purity: |
| Purified IgG was prepared from whole serum by affinity chromatography. |
| Form |
| Supplied as a liquid in PBS with 0.02% sodium azide. |
| Specificity: |
| Recognizes endonuclease G (endoG), a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis endoG i reported to act independently of caspases and may represent a caspase independent apoptotic pathway initiated from the mitochondria. Species Crossreactivity: Human. Expected crossreactivity from sequence similarity mouse, rat. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Li Ly, et al., (2001) Nature 412:95-99.
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