Technical Data
ERAS (GTPase ERas, E-Ras, Embryonic Stem Cell-expressed Ras, HRAS2, HRASP)
The human ERAS gene was initially reported as a processed pseudogene (HRasp, Ha-Ras2), with several base substitutions in coding regions, but was more recently described as potentially encoding a functional human ERAS protein. The ERAS oncogene supports the tumorigenic growth of embryonic stem cells by producing a constitutively active Ras protein. ERAS protein has less than 50 identity with H-Ras, another member of Ras family but the key domains essential for Ras function are highly conserved. The protein also contains amino-acid residues identical to those present in active mutants of Ras and causes oncogenic transformation in NIH 3T3 cells. Human ERAS gene is localized in the chromosomal region Xp11.23.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to aa178-195 (VETSAKTRQGVEEAFSLL) of human ERAS.
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Recognizes human ERAS. Species Crossreactivity: rat. Species sequence homology: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Takahashi, K. et al. Nature. 423:541-545 (2003). 2. Takashi, K. et al. Stem Cells. 23:1535-1540 (2005).