Technical Data
E2291-63
ERAS (GTPase ERas, E-Ras, Embryonic Stem Cell-expressed Ras, HRAS2, HRASP)
Description:
The human ERAS gene was initially reported as a processed pseudogene (HRasp, Ha-Ras2), with several base substitutions in coding regions, but was more recently described as potentially encoding a functional human ERAS protein. The ERAS oncogene supports the tumorigenic growth of embryonic stem cells by producing a constitutively active Ras protein. ERAS protein has less than 50 identity with H-Ras, another member of Ras family but the key domains essential for Ras function are highly conserved. The protein also contains amino-acid residues identical to those present in active mutants of Ras and causes oncogenic transformation in NIH 3T3 cells. Human ERAS gene is localized in the chromosomal region Xp11.23.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to aa178-195 (VETSAKTRQGVEEAFSLL) of human ERAS.
Purity:
Serum
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity:
Recognizes human ERAS. Species Crossreactivity: rat. Species sequence homology: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Takahashi, K. et al. Nature. 423:541-545 (2003). 2. Takashi, K. et al. Stem Cells. 23:1535-1540 (2005).