		Technical Data
E2291-75M	ERp57 (PDIA3, Protein disulfide isomerase family A, member 3, 58kD glucose-regulated protein, 58kD microsomal protein, Disulfide isomerase ER-60, ER60, ERp57, ERP57, ERp60, ERP60, ERp61, GRP57, GRP58, HsT17083, p58, P58, PI-PLC, Protein disulfide-isomeras

Description:
ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin
(lectin chaperones) via their proline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glucosylated side chains. ERp57 deletion impairs
posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and
leuteinizing-hormone-releasing hormone in the hippocampus (7).

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	9L798	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Recombinant human ERp57 (GRP58).

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

Specificity:

Recognizes human ERp57 at ~57kD. Species Crossreactivity: monkey, rat, hamster, rabbit, porcine, canine, guinea pig.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Herbert D.N. and Molinari M. (2007) Physiol Rev. 87:1377-1408. 2. Williams D.B. (2005) J Cell Sci. 119: 615-623 3. Maattanen P., et al. (2006) Biochem Cell Biol. 84:881-889. 4. Oliver J.D., et al. (1999) Mol Bio Cell. 10: 2573-2582. 5. Oliver J.D., et al. (1997) Science 275: 86-88. 6. Solda T., et al. (2006) J Biol Chem 281: 6219-6226. 7. Kimura T., et al. (2005) Biochem Biophys Research Communications. 331 (1): 224-230. 8. Chen, G., et al. (2002) Clin Cancer Res 8(7): 2298-2305. 9. Tan, P., et al. F. (2002) J Immunol 168(4): 1950-1960.