Technical Data
E2291-75N
Endoplasmic Reticulum Protein 57, CT (ERp57, GRP58, PDIA3, Protein Disulfide Isomerase Family A, Member 3 [Homo sapiens], HGNC:4606, ERp60, ERp61, GRP57, HsT17083, P58, PI-PLC, 58kD Microsomal Protein, Endoplasmic Reticulum P58, Glucose Regulated Protein,
Description:
Protein disulphide isomerase family A member 3 (PDIA3) is a 55kD protein of the endoplasmic reticulum. PDIA3 interacts with lectin chaperones calreticulin and calnexin, modulating folding of newly synthesized glycoproteins by promoting the formation of disulphide bonds.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:80,000
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Peptide sequence C-EEKPKKKKKAQEDL corresponding to the C-terminus of PDIA3 (NP_05304).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in tris-saline, pH 7.3, 0.02% sodium azide, 0.5% BSA.
Specificity:
Recognizes human protein disulphide isomerase family A, member 3 (PDIA3). Species sequence homology: rat, mouse, rabbit, hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Oliver, J. et al. (1997) Interaction of the thiol-dependent reductase ERp57 with nascent glycoproteins. Science 275: 86-88. 2. Bourdi, M. et al. (1995) cDNA cloning and baculovirus expression of the human liver endoplasmic reticulum P58: characterization as a protein disulfide isomerase isoform, but not as a protease or a carnitine acyltransferase. Arch. Biochem. Biophys. 323: 397-403.