Technical Data
Endoplasmic Reticulum Protein 57, CT (ERp57, GRP58, PDIA3, Protein Disulfide Isomerase Family A, Member 3 [Homo sapiens], HGNC:4606, ERp60, ERp61, GRP57, HsT17083, P58, PI-PLC, 58kD Microsomal Protein, Endoplasmic Reticulum P58, Glucose Regulated Protein,
Protein disulphide isomerase family A member 3 (PDIA3) is a 55kD protein of the endoplasmic reticulum. PDIA3 interacts with lectin chaperones calreticulin and calnexin, modulating folding of newly synthesized glycoproteins by promoting the formation of disulphide bonds.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:80,000
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Peptide sequence C-EEKPKKKKKAQEDL corresponding to the C-terminus of PDIA3 (NP_05304).
Purified by immunoaffinity chromatography.
Supplied as a liquid in tris-saline, pH 7.3, 0.02% sodium azide, 0.5% BSA.
Recognizes human protein disulphide isomerase family A, member 3 (PDIA3). Species sequence homology: rat, mouse, rabbit, hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Oliver, J. et al. (1997) Interaction of the thiol-dependent reductase ERp57 with nascent glycoproteins. Science 275: 86-88. 2. Bourdi, M. et al. (1995) cDNA cloning and baculovirus expression of the human liver endoplasmic reticulum P58: characterization as a protein disulfide isomerase isoform, but not as a protease or a carnitine acyltransferase. Arch. Biochem. Biophys. 323: 397-403.