Technical Data
E2296-05D
Endostatin
Description:
Endostatin (20-22kD), a cleaved product of the C-terminal domain of collagen XVIII, is an endogenous angiogenesis inhibitor. It was originally found in conditioned media from a murine endothelial tumor cell line, hemangioendothelioma. Endostatin inhibits endothelial cell migration (in vivo and in vitro) and induces endothelial cell apoptosis. It inhibits tumor growth and impairs blood vessel maturation in wound healing. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes.

Cellular Localization: Cytoplasmic and Nuclear

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b8k41Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.5mg/ml
Immunogen:
Purified recombinant mouse endostatin, expressed in insect Sf 21 cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity:
Species Crossreactivity: Crossreacts with Mouse.Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dixelius J et al. (2002) Cancer Res 62:1944-7. 2. Bloch W et al. (2000) FASEB J 14:2373-6. 3. Sasaki T et al. (2000) J Mol Biol 301:1179-90. 4. O'Reilly MS et al. (1997) Cell 88:277-85.