Technical Data
E2800
ENA78 (Epithelial Neutrophil Activating Peptide) Recombinant, Human
5ug
20ug
Growth Factors, Cytokines Storage: -20CShipping: RT
Epithelial Neutrophil Activating Peptide-78 (ENA-78) is a recently discovered chemoattractant and activator for neutrophils, belonging to the IL-8 subgroup of the C-X-C family of chemokines. Human ENA-78 is an 8.0kDa protein containing 74 amino acid residues.

Formulation: Lyophilized protein with no additives. The protein may appear as a haze or film which is difficult to see at the bottom of the vial.

Stability: The lyophilized protein, though stable at room temperature, is best stored desiccated below 0C. Reconstituted human ENA-78 should be stored in working aliquots at -20C.

Purity: 98% by SDS-PAGE and HPLC

Endotoxin: 0.1ng/ug

Reconstitution: The lyophilized ENA-78 is soluble in water and most aqueous buffers. The lyophilized protein can be reconstituted in water to a concentration of 0.1-1mg/ml. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.

Biological Activity: Human ENA-78 produces chemotaxis over a wide concentration range in an assay using human peripheral blood neutrophils. Significant chemotaxis was achieved using a concentration of 50ng/ml.

Responding Cells: Neutrophils

Concentration Range: 25-500ng/ml [for most in vitro applications]

AA Sequence: AAVLRELRCV CLQTTQGVHP KMISNLQVFA IGPQCSKVEV VASLKNGKEI CLDPEAPFLK KVIQKILDGG NKEN

Molecular Weight:
8.0kD
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.