Technical Data
E2900
Endostatin
Description:
Endostatin, which corresponds to the C-terminal fragment of collagen XVIII, is a potent inhibitor of endothelial cell proliferation, migration and angiogenesis. Recent study shows that endostatin induces tyrosine kinase activity and enhanced apoptosis in FGF-treated endothelial cells. Endostatin is currently being used in Phase I trails as an antitumor agent.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:2000. Detects endostatin in mouse liver cell lysates to which 50ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with E2900. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.

Recommended secondary antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG4i37Affinity Purified
SizeStorageShippingSourceHost
200ul-20°CBlue IceMouseMouse
Concentration:
Not Determined
Immunogen:
Full-length recombinant mouse Endostatin. Species Sequence Homology: rat.
Purity:
Puri ed by Thiophilic chromatography.
Form
Supplied as a liquid in 50mM sodium phosphate, pH 8.0, 0.05% sodium azide, before the addition of glycerol to 30%.
Specificity:
Recognizes mouse Endostatin, Mr 22kD, an unidenti ed protein is also weakly detected, Mr 54kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sauter, B.V., et al., (2000) Proc. Natl. Acad. Sci. USA 97:4802–4807. 2. Dhanabal, M., et al., (1999) J. Biol. Chem. 274:11,721–11,726.