Technical Data
E2900-02
Endostatin
Description:
Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during the normal tissue repair, and for the remodeling of the female reproductive organs (ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000-1:5000. Human and mouse endostatins are ~20kD.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Purified human endostatin
Purity:
Serum
Form
Supplied as a liquid in PBS, 0.1% BSA.
Specificity:
Recognizes human Endostatin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Peterson Te et al (1990) JBC 265:6104-6111 2. Forsgren m et al (1987) FEBS Lett. 213:254-260. 3. Malinowski DP et al (1984) Biochemistry 23:4243-4250. 4. O'Reilly MS et al (1994) Cell 79:315-328. 5. Sim BK et al (1997) Cancer Res. 57:1329-1334. 6. Wu Z et al (1997) BBRC 236:651.