Technical Data
E2900-03
Endostatin
Description:
Endostatin is a potent inhibitor of endothelial cell proliferation, migration and angiogenesis. Recent study shows that endostatin induces tyrosine kinase activity and enhanced apoptosis in FGF-treated endothelial cells. Endostatin is currently being used in Phase I trails as an antitumor agent.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:3200 (0.312ug/ml)
Optimal dilutions to be determined by the researcher.

AA Sequence:
Ala - Asp - Arg - Ala - Ala - Val - Pro - Ile - Val - Asn - Leu - Lys - Asp - Glu - Leu - Leu - Phe - Pro - Ser - Trp - Glu - Ala - Leu - Phe - Ser - Gly - Ser - Glu - Gly - Pro - Leu - Lys - Pro - Gly - Ala - Arg - Ile - Phe - Ser - Phe - Asp - Gly - Lys - Asp - Val - Leu - Arg - His - Pro - Thr - Trp - Pro - Gln - Lys - Ser - Val - Trp - His - Gly - Ser - Asp - Pro - Asn - NH2

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Synthetic peptide corresponding to aa52-114 of human Endostatin.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes human Endostatin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.