Technical Data
Media
Endothelial Basal Medium MCDB 107 (Powder)
E3000
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•2H2O111
Cupric Sulfate•5H2O0.0002497
Ferric Nitrate•9H2O1.39
Potassium Chloride149
Potassium phosphate mono408
Magnesium Sulfate120.38
Manganese Sulfate•5H2O0.00241
SodiumChloride6599.2
Sodium Selenite•5H2O0.00789
Sodium Metasilicate•9H2O0.1421
Ammonium Molybdate•4H2O0.0012359
Ammonium Metavandate0.000585
Nickel Chloride•6H2O0.00011885
Stannous Chloride•2H2O0.0001128
Zinc Sulfate•7H2O0.14375
Amino Acids:
L-Alanine8.9
L-Arginine174.2
L-Asparagine13.21
L-Aspartic Acid13.31
L-Cysteine6.11
L-Glutamic Acid14.71
L-Glutamine365
Glycine22.53
L-Histidine15.52
L-Isoleucine3.94
L-Leucine13.12
L-Lysine29.24
L-Methionine4.48
L-Phenylalanine4.96
L-Proline34.53
L-Serine10.51
L-Threonine11.91
L-Tryptophan2.042
L-Tyrosine5.436
L-Valine11.72
Vitamins:
Biotin0.007329
D-Pantothenate, Ca0.2383
Choline Chloride13.96
Folinic Acid, Ca0.0006016
Myo-Inositol18.02
Nicotinamide6.105
Pyridoxine•HCl0.02056
Riboflavin0.11292
Thiamine•HCl0.3373
Vitamin B120.13554
Other:
Adenine1.35
D-Glucose720.8
HEPES Buffer7149
Lipoic Acid0.002063
Phenol Red, Na1.129
Putrescine•2HCl0.0001611
Sodium Pyruvate110
Thymidine0.07266
Total16.2g/liter
Basal Medium for the growth of nonendothelial mesenchymal cells. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed).


Directions per Liter: Dissolve 16.2g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Off-white, homogeneous, free flowing powder

Solubility: Yellow, clear, complete

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.