Technical Data

Media

Endothelial Basal Medium MCDB 107 (Powder)

E3000

Cell Culture Grade

Storage RT/4°C Shipping RT

Components shown as mg/liter


Inorganic Salts:
Ammonium Metavandate	0.000585
Ammonium Molybdate•4H2O	0.0012359
Calcium Chloride•2H2O	111
Cupric Sulfate•5H2O	0.0002497
Ferric Nitrate•9H2O	1.39
Magnesium Sulfate	120.38
Manganese Sulfate•5H2O	0.00241
Nickel Chloride•6H2O	0.00011885
Potassium Chloride	149
Potassium phosphate mono	408
Sodium Chloride	6599.2
Sodium Metasilicate•9H2O	0.1421
Sodium Selenite•5H2O	0.00789
Stannous Chloride•2H2O	0.0001128
Zinc Sulfate•7H2O	0.14375

Amino Acids:
L-Alanine	8.9
L-Arginine	174.2
L-Asparagine	13.21
L-Aspartic Acid	13.31
L-Cysteine	6.11
L-Glutamic Acid	14.71
L-Glutamine	365
Glycine	22.53
L-Histidine	15.52
L-Isoleucine	3.94
L-Leucine	13.12
L-Lysine	29.24
L-Methionine	4.48
L-Phenylalanine	4.96
L-Proline	34.53
L-Serine	10.51
L-Threonine	11.91
L-Tryptophan	2.042
L-Tyrosine	5.436
L-Valine	11.72

Vitamins:
Biotin	0.007329
Choline Chloride	13.96
Vitamin B12	0.13554
Folinic Acid, Ca	0.0006016
Myo-Inositol	18.02
Nicotinamide	6.105
D-Pantothenate, Ca	0.2383
Pyridoxine•HCl	0.02056
Riboflavin	0.11292
Thiamine•HCl	0.3373

Other:
Adenine	1.35
Thymidine	0.07266
D-Glucose	720.8
HEPES Free Acid	7149
Lipoic Acid	0.002063
Phenol Red, Na	1.129
Putrescine•2HCl	0.0001611
Sodium Pyruvate	110

Total	16.2g/liter

Basal Medium for the growth of nonendothelial mesenchymal cells. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed).

Directions per Liter: Dissolve 16.2g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Off-white, homogeneous, free flowing powder

Solubility: Yellow, clear, complete

pH: As Reported

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.