Technical Data

Media

Endothelial Basal Medium MCDB 131 w/o Phenol Red, Cysteine, Methionine (Powder)

E3000-01

Cell Culture Grade

Storage RT/4°C Shipping RT

Components shown as mg/L

Inorganic Salts:
Ammonium Metavanadate	0.0006
Ammonium Molybdate•4H2O	0.0037
Calcium Chloride•2H2O	235
Cupric Sulfate•5H2O	0.0012
Ferrous Sulfate•7H2O	0.283
Magnesium Sulfate•7H2O	2464
Manganese Sulfate•H20	0.0002
Nickel Chloride•6H2O	7.1
Potassium Chloride	298
Sodium Chloride	6430
Sodium Meta Silicate•9H2O	2.8
Sodium Phosphate Dibasic•7H2O	134
Zinc Sulfate•H2O	0.0003
Amino Acids:
L-Alanine	2.7
L-Arginine Hydrochloride	63.2
L-Asparagine•H2O	15
L-Aspartic Acid	13.3
L-Cysteine•2HCl•H2O	absent
L-Glutamic Acid	4.4
Glycine	2.3
L-Histidine Hydrochloride•H2O	42
L-Isoleucine	66
L-Leucine	131
L-Lysine Hydrochloride	182
L-Methionine	absent
L-Phenylalanine	33
L-Proline	11.5
L-Serine	32
L-Threonine	12
L-Tryptophan	4.1
L-Tyrosine	18.1
L-Valine	117
Vitamins:
Biotin	0.0073
Choline Chloride	14
Vitamin B12	0.0136
Folinic Acid Calcium	0.6
i-Inositol	7.2
Niacinamide	6.1
D-Calcium Pantothenate	12
Pyridoxine Hydrochloride	2.1
Riboflavin	0.0038
Thiamine Hydrochloride	3.4
Other Components:
Adenine	0.135
Thymidine	0.024
D-Glucose	1000
Lipoic Acid	0.0021
Phenol Red	absent
Putrescine•2HCl	0.0002
Selenious Acid	0.0038
Sodium Pyruvate	110
Total:	11.48g/L

MCDB 131 was developed by Knedler and Ham as a reduced serum-supplemented medium for the culture of human microvascular endothelial cells. This formulation has been reported to support clonal growth of human omental microvascular cells when supplemented with 0.7% dialyzed FBS, 10ng/mL EGF, and 1ug/mL hydrocortisone.

Appearance:
White to off-white, homogenous, free-flowing powder

Solubility:
Colorless, clear, complete

pH:
As reported

Endotoxin: 1EU/ml

Directions per Liter: Dissolve 11.48g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application reference: Lopez-Quintero, S.V. et al., (2011) Invest Ophthalmol Vis Sci. 52: 1171–1180. 1. Knedler A and Ham RG. (1987) In Vitro Cell. Develop. Biol. 23:481-491.