Technical Data
Media
Endothelial Basal Medium MCDB 131 (Powder)
E3000-01B
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/L
Inorganic Salts:
Ammonium Metavanadate0.0006
Ammonium Molybdate•4H2O0.0037
Calcium Chloride•2H2O235
Cupric Sulfate•5H2O0.0012
Ferrous Sulfate•7H2O 0.283
Magnesium Sulfate•7H2O2464
Manganese Sulfate•H200.0002
Nickel Chloride•6H2O0.000071
Potassium Chloride298
Sodium Chloride6430
Sodium Meta Silicate•9H2O2.8
Sodium Phosphate Dibasic•7H2O134
Zinc Sulfate•H2O0.0003
Amino Acids:
L-Alanine2.7
L-Arginine Hydrochloride63.2
L-Asparagine•H2O15
L-Aspartic Acid13.3
L-Cysteine•2HCl.•H2O35
L-Glutamic Acid4.4
Glycine2.3
L-Histidine Hydrochloride•H2O42
L-Isoleucine66
L-Leucine131
L-Lysine Hydrochloride182
L-Methionine15
L-Phenylalanine33
L-Proline11.5
L-Serine32
L-Threonine12
L-Tryptophan4.1
L-Tyrosine18.1
L-Valine117
Vitamins:
Biotin0.0073
Choline Chloride14
Vitamin B120.0136
Folinic Acid Calcium0.6
i-Inositol7.2
Niacinamide6.1
D-Calcium Pantothenate12
Pyridoxine Hydrochloride2.1
Riboflavin0.0038
Thiamine Hydrochloride3.4
Other Components:
Adenine0.135
Thymidine0.024
D-Glucose1000
Lipoic Acid0.0021
Phenol Red12.4
Putrescine•2HCl0.0002
Selenious Acid0.0038
Sodium Pyruvate110
Total:11.53g/L
MCDB 131 was developed by Knedler and Ham as a reduced serum-supplemented medium for the culture of human microvascular endothelial cells. This formulation has been reported to support clonal growth of human omental microvascular cells when supplemented with 0.7% dialyzed FBS, 10ng/mL EGF, and 1ug/mL hydrocortisone.


Directions per Liter: Dissolve 11.53g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Pale orange to orange, homogeneous, free-flowing powder

Solubility: Pink to orange, clear, complete

pH: As reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application reference: Di Meglio, F. et al., (2010) Italian J. of Anatomy and Embryology 115: 71-78. 1. Knedler A and Ham RG. (1987) In Vitro Cell. Develop. Biol. 23:481-491