Technical Data
Media
Endothelial Basal Medium MCDB 120 (Powder)
E3000-02
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/L
Inorganic Salts:
Ammonium Metavanadate 0.0006
Ammonium molybdate•4H2O 0.00371
Calcium Chloride•2H2O235.2
Cupric Sulfate•5H2O0.0025
Ferrous Sulfate•7H2O0.834
Magnesium Sulfate•anhydrous120.4
Manganese Sulfate•H200.000241
Nickel Chloride•6H2O0.0000713
Potassium Chloride298.2
Sodium Chloride6428.4
Sodium Meta Silicate•9H2O2.842
Sodium Phosphate Dibasic71
Zinc Sulfate•7H2O0.08625
Amino Acids:
L-Alanine2.67
L-Arginine Hydrochloride210.67
L-Asparagine•H2O15.01
L-Aspartic Acid13.31
L-Cysteine•HCl•H2O35.12
L-Glutamic Acid4.41
L-Glutamine1461.5
Glycine2.25
L-Histidine Hydrochloride•H2O41.93
L-Isoleucine65.58
L-Leucine131.2
L-Lysine Hydrochloride181.65
L-Methionine29.84
L-Phenylalanine33.04
L-Proline11.51
L-Serine31.53
L-Threonine35.73
L-Tryptophan4.08
L-Tyrosine•2Na•2H2O22.52
L-Valine117.15
Vitamins:
Biotin0.00733
Choline Chloride13.96
Vitamin B120.0136
Folinic Acid Calcium0.5115
i-Inositol10.09
Nicotinamide6.105
D-Pantothenic Acid (hemi Ca)47.7
Pyridoxine Hydrochloride2.056
Riboflavin0.0038
Thiamine Hydrochloride3.373
Other Components:
Adenine HCl0.1716
Thymidine0.0242
D-Glucose1000
Lipoic Acid0.0021
Phenol Red1.2421
Putrescine•2HCl0.0001611
Sodium Pyruvate110
Sodium Selenite 0.0052
Total:10.803g/L
MCDB media were designed for the low-protein or serum-free growth of specific cell types using hormones, growth factors, trace elements or low levels of dialyzed fetal bovine serum protein (FBSP). Each MCDB medium was formulated (qualitatively and quantitatively) to provide a defined and optimally balanced nutritional environment that selectively promoted growth of a specific cell type.

Appearance:
Off-white to orange, homogeneous, free flowing powder

Solubility:
Pink, clear, complete

pH:
As reported

Endotoxin: 1EU/ml


Directions per Liter: Dissolve 10.80g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.