Technical Data
E3010
Endothelial Cell Growth Supplement (ECGS)
50mg
10x15mg
10x15mg
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Technical Data |
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E3010 |
Endothelial Cell Growth Supplement (ECGS) |
50mg 10x15mg |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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ECGS is mitogenic for many types of cells (i.e. mammalian, avian and human endothelial cells, smooth muscle cells, keratinocytes, melanocytes and hybridomas) under reduced or serum-free conditions. It often fully substitutes for feeder layers in culture of fastidious cells. Bovine hypothalamic protein extract, is rich in cell growth and attachment factors. Range for Optimal Activity: Use in culture at 10-100ug/ml Biological Activity: 15ug/ml supports 4.6-fold growth of fetal bovine heart endothelial cells cultured for 5 days in 50% RPMI/50% DMEM (RD) medium, 2% FBS. Results may vary depending on cell line used. Storage and Stability: Lyophilized powder is stable for 12 months at -20°C. Reconstituted product is stable for 3 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
Source: Bovine hypothalamus. Purity: Filtered through a 0.2um membrane and packaged aseptically. Form: Supplied as a lyophilized powder from sterile water containing trace amounts of sodium chloride, streptomycin sulfate. Reconstitute with sterile medium, physiological buffer or water. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Hoshi, H., McKeehan, W.L., (1986) In Vitro Cell. Devel. Biol. 22:51-56. 2. Maciag, T., et al., (1979) PNAS USA 76:5574. 3. Maciag, T., et al., (1986) Proc. Throm. Hemostatis 7:167. 4. Pintus, C., et al., (1983) J. Immunol. Methods 61:195.
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