Technical Data
Endothelial Cell Growth Supplement (ECGS)
Molecular Biology Storage: -20░CShipping: Blue Ice
ECGS is mitogenic for many types of cells (i.e. mammalian, avian and human endothelial cells, smooth muscle cells, keratinocytes, melanocytes and hybridomas) under reduced or serum-free conditions. It often fully substitutes for feeder layers in culture of fastidious cells. Bovine hypothalamic protein extract, is rich in cell growth and attachment factors.

Range for Optimal Activity:
Use in culture at 10-100ug/ml

Biological Activity:
15ug/ml supports 4.6-fold growth of fetal bovine heart endothelial cells cultured for 5ádays in 50% RPMI/50% DMEM (RD) medium, 2% FBS. Results may vary depending on cell line used.

Storage and Stability:
Lyophilized powder is stable for 12 months at -20░C. Reconstituted product is stable for 3 months at -20░C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Bovine hypothalamus.
Purity: Filtered through a 0.2um membrane and packaged aseptically.
Form: Supplied as a lyophilized powder from sterile water containing trace amounts of sodium chloride, streptomycin sulfate. Reconstitute with sterile medium, physiological buffer or water.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Hoshi, H., McKeehan, W.L., (1986) In Vitro Cell. Devel. Biol. 22:51-56. 2. Maciag, T., et al., (1979) PNAS USA 76:5574. 3. Maciag, T., et al., (1986) Proc. Throm. Hemostatis 7:167. 4. Pintus, C., et al., (1983) J. Immunol. Methods 61:195.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.