		Technical Data
E3010-36	Endothelial Lipase (EL)

Description:
Endothelial lipase (EL) is a member of the triglyceride lipase gene family. It functions primarily as a phospholipase and has low triglyceride lipase activity. It has been shown to be a major genetic determinant for the concentration, structure, and metabolism of high-density lipoprotein, which protects against atherosclerosis (1,2). It was originally cloned from endothelial cells and was found to be expressed in a distinct and complementary tissue-restricted fashion, with high-level expression in the liver, placenta, lung, ovary, and macrophage(3). The wide spread distribution of this protein suggests that it plays a general role in lipid metabolism. Immunohistochemical studies demonstrate that EL is expressed in infiltrating cells such as macrophages within atheromatous plaques, in addition to endothelial and smooth muscle cells in non-atherosclerotic coronary arteries. Furthermore, EL expression is detected in the neovasculature within atheromatous plaques in atherosclerotic coronary arteries, indicating that EL may have unique functional roles in atherosclerosis.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 3ug/ml
Immunohistochemistry (FFPE): 2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Western Blot: HepG2 cell lysate
Immunohistochemistry: Paraffin-embedded rat kidney sections.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
1 vial		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.6mg/ml

Immunogen:

Synthetic peptide corresponding to aa19-32, AGSPVPFGPEGRLE of human endothelial lipase.

Purity:

Purified by immunoaffinity chromatography

Form

Supplied as a liquid in TBS, pH 7.4, 0.5mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Specificity:

Recognizes human Endothelial Lipase at ~57kD. Species Crossreactivity: mouse, rat, sheep and porcine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1 Ishida, T., Choi, S., Kundu, R.K., et al., J. Clin. Invest. 111(3): 347-355 (2003). 2. Ma, K., Cilingiroglu, M., Otvos, J.D., et al., Proc. Natl. Acad. Sci. USA 100(5): 2748-2753 (2003). 3. Hirata, K., Dichek, H.L., Cioffi, J.A., et al., J. Biol. Chem. 274(20): 14,170-14,175 (2003). 4. Azumi, H., Hirata, K., Ishida, T., et al., Cardiovascular Res. 58: 647-654 (2003).