Technical Data
E3010-36A
Endothelial Lipase (EL)
Description:
Endothelial lipase (EL) is a novel member of the lipoprotein lipase gene family. It is synthesized by endothelial cells and functions at the site of synthesis. EL most likely plays a physiological role in modulating lipoprotein metabolism. Overexpressed EL has been shown to reduce HDL cholesterol levels, whereas an EL deficiency appears to be effective in elevating HDL cholesterol levels in animals.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500. Recognizes a band at ~57kD, representing the mature,
non-glycosylated form of endothelial lipase protein. Another band is seen at ~45kD,
representing the proteolytic form of the protein.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
An N-terminal synthetic peptide made to the human endothelial lipase protein sequence.
Purity:
Serum
Form
Supplied as a liquid (antiserum)
Specificity:
Recognizes human endothelial lipase. Does not appear to recognize murine endothelial lipase.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.