Technical Data
E3010-36F
Endothelial Lipase (LIPG, Endothelial Cell-derived Lipase, EDL, EL, UNQ387/PRO719)
Description:
Endothelial lipase (EL) is a member of the triglyceride lipase gene family. It functions primarily as a phospholipase and has low triglyceride lipase activity. It was originally cloned from endothelial cells and found to be expressed in high level in the liver, placenta, lung, ovary, and macrophage. The wide spread distribution of this protein suggests it plays a general role in lipid metabolism. Human endothelial lipase has an estimated molecular weight of 57kD.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Immunohistochemistry (Frozen and paraffin): 5-20ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa22 from human Endothelial Lipase.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Specificity:
Recognizes human Endothelial Lipase at ~57kD. Species Crossreactivity: mouse, rat, porcine and sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.