Technical Data
E3098-25C
Endothelin 1 (EDN1, Endothelin-1, ET1, HDLCQ7, PPET1, Preproendothelin-1)
Description:
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21aa peptides exists in at least three isoforms, ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart and liver.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Radioimmunoassay. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Paraffin sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
20ul-20CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Human Endothelin 1.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with sterile PBS.
Specificity:
Recognizes human Endothelin 1. Species Crossreactivity: porcine and domestic ruminants (bovine, goat, sheep, etc).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Acosta TJ, Berisha B, Ozawa T, Sato K, Schams D, Miyamoto A. Evidence for a local endothelin-angiotensin-atrial natriuretic peptide system in bovine mature follicles in vitro: effects on steroid hormones and prostaglandin secretion.Biol Reprod. 1999 Dec;61(6):1419-25. 2. Acosta TJ, Ozawa T, Kobayashi S, Hayashi K, Ohtani M, Kraetzl WD, Sato K, Schams D, Miyamoto A. Periovulatory changes in the local release of vasoactive peptides, prostaglandin f(2alpha), and steroid hormones from bovine mature follicles in vivo.Biol Reprod. 2000 Nov;63(5):1253-61.