Technical Data
E3098-28
Endothelin 1
Description:
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms, ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart and liver.

Applications:
Suitable for use in ELISA and RIA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Source:
Ascites

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG11.B.759Affinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human Endothelin 1.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.0, before the addition of glycerol to 40%. No preservative added.
Specificity:
Recognizes human Endothelin 1. Crossreactivities determined by competitive RIA. Concentrations (pg/tube) to give 50% B/Bo: Endothelin 1 (human) - 20; Endothelin 2 (human) - 2; Endothelin 3 (human) - 1000; Big Endothelin (human) - 2; Endothelin C-terminal hexapeptide - 0; Atrial natriuretic peptide (human) - 0; Angiotensin I - 0; Angiotensin II - 0.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.