Technical Data
E3098-29C
Endothelin 1
Description:
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms, ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart and liver.

Applications:
Suitable for use in Immunohistochemistry (frozen) and the development of IRMA assays for endothelin ET-1. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Affinity Constant: 4x10e10

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19L199Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic human Endothelin-1 corresponding to C-Terminal fragment.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 10mM sodium azide.
Specificity:
Recognizes Endothelin 1. Cross-reactivity: Endothelin 1: 100%; Endothelin 3: <10%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.