		Technical Data
E3098-29E	Endothelin 1

Description:
Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms, ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart and liver.

Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry, Western Blot and RIA. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Paraffin): 1:300
Immunohistochemistry (Frozen): 1:250
RIA: 1:25,000. Use to concentrate ET-1 in solution, e.g. serum/plasma, milk, urine.
Optimal dilutions to be determined by the researcher.

Positive Control:
Human bowel

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	6D367	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~2.5mg/ml

Immunogen:

Purified human ET-1 (endothelin-1)

Purity:

Purified by affinity chromatography

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

Specificity:

Recognizes human ET-1 (epitope aa8-16). Crossreacts slightly with related peptides. Species Crossreactivity: rat and sheep

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.